Recombinant human PDE2A protein (Active) (ab271676)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 33% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PDE2A protein (Active)
See all PDE2A proteins and peptides -
Biological activity
Specific Activity: ≥ 3442 pmol/min/µg.Unit Definition:One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE2A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
Purity
>= 33 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
132 kDa including tags -
Tags
GST tag N-Terminus -
Additional sequence information
Full length, with N-terminal GST-tag and N-terminal thrombin cleavage site.
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Preparation and Storage
- 5''-cyclic phosphodiesterase
- cGMP dependent 3'5' cyclic phosphodiesterase
- cGMP stimulated phosphodiesterase 1
Images
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Functional Studies - Recombinant human PDE2A protein (Active) (ab271676)
Specific activity of ab271676 was ≥ 3442 pmol/min/µg.
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SDS-PAGE - Recombinant human PDE2A protein (Active) (ab271676)SDS-PAGE analysis of 2 µg ab271676.
