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Recombinant Human RSV glycoprotein (His tag) (ab206016)

Recombinant Human RSV glycoprotein (His tag) (ab206016)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 90% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant Human RSV glycoprotein (His tag)
  • Purity

    > 90 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells
  • Accession

    P20895
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      HKVTLTTAIIQDATSQIKNTTPTYLTQDPQLGISFSNLSEITSQTTTILA STTPGVKSNLQPTTVKTKNTTTTQTQPSKPTTKQRQNKPPNKPNNDFHFE VFNFVPCSICSNNPTCWAICKRIPNKKPGKKTTTKPTKKPTFKTTKKDHK PQTTKPKEVPTTKPTEEPTINTTKTNIITTLLTNNTTGNPKLTSQMETFH STSSEGNLSPSQVSTTSEHPSQPSSPPNTTR
    • Predicted molecular weight

      26 kDa including tags
    • Amino acids

      67 to 297
    • Tags

      His tag C-Terminus
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab206016 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Additional notes

      This product is stable after storage at:

      • -20°C to -70°C for 12 months in lyophilized state;
      • -70°C for 3 months under sterile conditions after reconstitution.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 100% PBS

      5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

    General Info

    • Alternative names

      • Attachment glycoprotein
      • Attachment glycoprotein G
      • Major surface glycoprotein G
      • Membrane-bound glycoprotein
      • mG
      see all
    • Relevance

      This protein of 86 kDa is the major surface glycoprotein G of Human respiratory syncytial virus. Glycoprotein G is the attachment protein of respiratory syncytial virus. RSV is a 100-350 nm enveloped virus belonging to the Paramyxoviridae, genus, Pneumovirus, that causes minor respiratory infections in adults and bronchitis and bronchopneumonia in children. Its genome consists of single stranded, negative sense RNA that encodes three envelope glycoproteins, a small hydrophobic (SH) protein of unknown function, a major glycoprotein (G) known as the attachment protein, and a fusion (F) protein.
    • Cellular localization

      Virion membrane.

Images

  • SDS-PAGE - Recombinant Human RSV glycoprotein (ab206016)
    SDS-PAGE - Recombinant Human RSV glycoprotein (ab206016)

    SDS-PAGE analysis of reduced ab206016 and staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 46-90 kDa in SDS-PAGE due to glycosylation.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab206016? Please let us know so that we can cite the reference in this datasheet.

    ab206016 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • -20°C to -70°C for 12 months in lyophilized state;
    • -70°C for 3 months under sterile conditions after reconstitution.

    Images

    • SDS-PAGE - Recombinant Human RSV glycoprotein (ab206016)
      SDS-PAGE - Recombinant Human RSV glycoprotein (ab206016)

      SDS-PAGE analysis of reduced ab206016 and staining overnight with Coomassie Blue. DTT-reduced Protein migrates as 46-90 kDa in SDS-PAGE due to glycosylation.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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