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Recombinant human PTP1B protein (Active) (ab51277)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human PTP1B protein (Active)
    See all PTP1B proteins and peptides

  • Biological activity

    Specific activity is > 10,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.


    Activity Assay:

    1. Prepare a 180ul assay buffer into a suitable container: The concentrations are 11mM Tris-HCl pH7.5, 55mM NaCl, 2.2mM DTT, 1.1mM MnCl2, 1.1mM EDTA, 33mM pNPP.
    2. Equilibrate to 37? and monitor the A405nm until value is constant using a spectrophotometer.
    3. Add 20ul of recombinant PTP-1B protein 12.5ug/ml in 0.1% BSA/DW.
    4. Record the absorbance A405nm for 5 minutes.

  • Purity

    > 95 % SDS-PAGE.
    This protein was purified by conventional column chromatographic techniques.

  • Expression system

    Escherichia coli

  • Accession

    P18031

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N

    • Predicted molecular weight

      37 kDa

    • Amino acids

      1 to 321

    • Additional sequence information

      (NP_002818)

Preparation and Storage

  • Alternative names

    • PTP1B
    • Non receptor tyrosine phosphatase 1
    • Protein phosphotyrosylphosphatase 1B
    • Protein tyrosine phosphatase 1B
    • Protein tyrosine phosphatase non receptor type 1
    • Protein tyrosine phosphatase placental
    • Protein-tyrosine phosphatase 1B
    • PTN1_HUMAN
    • PTP 1B
    • PTP-1B
    • PTPN 1
    • PTPN1
    • Tyrosine protein phosphatase non receptor type 1
    • Tyrosine-protein phosphatase non-receptor type 1
    see all

  • Function

    May play an important role in CKII- and p60c-src-induced signal transduction cascades.

  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
    Contains 1 tyrosine-protein phosphatase domain.

  • Post-translational
    modifications

    Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.

  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human PTP1B protein (ab51277)
    SDS-PAGE - Recombinant human PTP1B protein (ab51277)

    3ug of reduced ab51277 on SDS-PAGE, stained with Coomassie Blue.

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