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Signal Transduction Protein Phosphorylation Tyrosine Phosphatases

Anti-SHP2 antibody [EPR17828-47] (ab187039)

Price and availability

307 566 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-SHP2 antibody [EPR17828-47] (ab187039)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17828-47] to SHP2
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-SHP2 antibody [EPR17828-47]
    See all SHP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR17828-47] to SHP2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 whole cell lysate; Mouse brain and heart lysate; Rat brain lysate. IP: Mouse heart lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17828-47
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Phosphatases
    • Neuroscience
    • Sensory System
    • Auditory system

Images

  • Western blot - Anti-SHP2 antibody [EPR17828-47] (ab187039)
    Western blot - Anti-SHP2 antibody [EPR17828-47] (ab187039)
    All lanes : Anti-SHP2 antibody [EPR17828-47] (ab187039) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15585863).

  • Immunoprecipitation - Anti-SHP2 antibody [EPR17828-47] (ab187039)
    Immunoprecipitation - Anti-SHP2 antibody [EPR17828-47] (ab187039)

    SHP2 was immunoprecipitated from 0.35 mg mouse heart lysate with ab187039 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187039 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Mouse heart lysate 10 µg (Input).
    Lane 2: ab187039 IP in mouse heart lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187039 in mouse heart lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 15 seconds.

  • Anti-SHP2 antibody [EPR17828-47] (ab187039)
    Anti-SHP2 antibody [EPR17828-47] (ab187039)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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