Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (ab220561)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: ELISA, Flow Cyt, Functional Studies, SDS-PAGE
Preparation and Storage
- CD155
- CD155 antigen
- FLJ25946
Images
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Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (ab220561)Immobilized Human CD155, Fc Tag at 5 µg/mL (100 µL/well) can bind Biotinylated Human TIGIT, His Tag with a linear range of 5-78 ng/mL.
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SDS-PAGE - Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (ab220561)SDS-PAGE analysis of reduced ab220561 stained overnight with Coomassie Blue.
The protein migrates as 95-105 kDa on a SDS-PAGE gel under reducing conditions due to glycosylation.
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Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (ab220561)FACS assay shows that recombinant Human CD155, Fc Tag can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 µg/ml. -
Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (ab220561)Loaded ab220561 on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.23 μM as determined in BLI assay (ForteBio Octet Red96e).
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Functional Studies - Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (ab220561)FACS analysis shows that the binding of Human CD155, Fc Tag to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 µg/ml. The IC50 is 0.09422 µg/ml.
