Recombinant human Poliovirus Receptor/PVR protein (Fc Chimera Active) (ab220469)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
- CD155
- CD155 antigen
- FLJ25946
Images
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FACS analysis shows that the binding of Human CD155, mouse IgG2a Fc Tag to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 µg/ml. The IC50 is 0.1413 µg/ml.
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FACS assay shows that recombinant Human CD155, mouse IgG2a Fc Tag can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 µg/ml.
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SDS-PAGE analysis of ab220469 stained overnight with Coomassie Blue.
Lane 1: Reducing conditions.
Lane 2: Non-reducing conditions.
The protein migrates as 70-100 kDa under reducing conditions and 130-160 kDa under non-reducing conditions on SDS-PAGE gel.
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Immobilized Human TIGIT, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 0.1-4 ng/ml.
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Immobilized Human DNAM-1, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220469 with a linear range of 1-16 ng/ml.