Recombinant human PLA2G1B protein (ab191680)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Please see notes section.
pH: 7.4
Constituents: 0.58% Sodium chloride, 0.41% Sodium acetate
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%.
ab191680 was lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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SDS-PAGE - Recombinant human PLA2G1B protein (ab191680)
DTT-reduced SDS-PAGE analysis of ab191680 stained overnight with Coomassie Blue. DTT-reduced protein migrates at 19 kDa in SDS-PAGE.
