Recombinant human PDGFR alpha (mutated D842V) protein (Active) (ab271685)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 84% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PDGFR alpha (mutated D842V) protein (Active)
See all PDGFR alpha proteins and peptides -
Biological activity
Specific Activity: ≥14 pmol/min/µg.
Assay Conditions: PDGFRa (D842V) reactions was done in 25 µl containing 40 mM Tris, pH 7.4, 20 mM MgCl2, 0.1 % BSA, 3 mM DTT, 0.2 mg/ml Poly (Glu, Tyr) and 20 µM ATP at 30°C for 45 min. The amount of ATP transferred to the substrate was measured using ADP-Glo reagents.
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Purity
>= 84 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
88 kDa including tags -
Amino acids
550 to 1089 -
Modifications
mutated D842V -
Tags
GST tag N-Terminus
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Preparation and Storage
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Alternative names
- Alpha-type platelet-derived growth factor receptor
- CD140 antigen-like family member A
- CD140a
see all -
Function
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity. -
Tissue specificity
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue. -
Involvement in disease
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant human PDGFR alpha (mutated D842V) protein (Active) (ab271685)
Specific activity of ab271685 was ≥14 pmol/min/µg.
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SDS-PAGE - Recombinant human PDGFR alpha (mutated D842V) protein (Active) (ab271685)SDS-PAGE analysis of ab271685.
