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Recombinant human PDGFR alpha (mutated D842V) protein (Active) (ab271685)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: >= 84% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human PDGFR alpha (mutated D842V) protein (Active)
    See all PDGFR alpha proteins and peptides

  • Biological activity

    Specific Activity: ≥14 pmol/min/µg.

    Assay Conditions: PDGFRa (D842V) reactions was done in 25 µl containing 40 mM Tris, pH 7.4, 20 mM MgCl2, 0.1 % BSA, 3 mM DTT, 0.2 mg/ml Poly (Glu, Tyr) and 20 µM ATP at 30°C for 45 min. The amount of ATP transferred to the substrate was measured using ADP-Glo reagents.

  • Purity

    >= 84 % SDS-PAGE.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    P16234

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      88 kDa including tags

    • Amino acids

      550 to 1089

    • Modifications

      mutated D842V

    • Tags

      GST tag N-Terminus

Preparation and Storage

  • Alternative names

    • Alpha-type platelet-derived growth factor receptor
    • CD140 antigen-like family member A
    • CD140a
    • CD140a antigen
    • MGC74795
    • PDGF alpha chain
    • PDGF Receptor alpha
    • PDGF-R-alpha
    • PDGFR 2
    • PDGFR alpha
    • PDGFR2
    • PDGFRA
    • PDGFRA/BCR fusion
    • PGFRA_HUMAN
    • Platelet derived growth factor receptor
    • Platelet derived growth factor receptor 2
    • Platelet derived growth factor receptor alpha
    • Platelet derived growth factor receptor alpha polypeptide
    • Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein
    • RHEPDGFRA
    see all

  • Function

    Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.

  • Tissue specificity

    Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue.

  • Involvement in disease

    Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA).

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human PDGFR alpha (mutated D842V) protein (Active) (ab271685)
    Functional Studies - Recombinant human PDGFR alpha (mutated D842V) protein (Active) (ab271685)

    Specific activity of ab271685 was ≥14 pmol/min/µg.

  • SDS-PAGE - Recombinant human PDGFR alpha (mutated D842V) protein (Active) (ab271685)
    SDS-PAGE - Recombinant human PDGFR alpha (mutated D842V) protein (Active) (ab271685)
    SDS-PAGE analysis of ab271685.

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