Recombinant human PDE2A protein (ab198457)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 88% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PDE2A protein
See all PDE2A proteins and peptides -
Biological activity
Specific Activity: ≥ 7577 pmol/min/μg
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 μM cAMP, and ab198457 at 37ºC for 20 min. Quantified by 5-nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
Purity
> 88 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GQACGHSILCRSQQYPAARPAEPRGQQVFLKPDEPPPPPQPCADSLQDAL LSLGSVIDISGLQRAVKEALSAVLPRVETVYTYLLDGESQLVCEDPPHEL PQEGKVREAIISQKRLGCNGLGFSDLPGKPLARLVAPLAPDTQVLVMPLA DKEAGAVAAVILVHCGQLSDNEEWSLQAVEKHTLVALRRVQVLQQRGPRE APRAVQNPPEGTAEDQKGGAAYTDRDRKILQLCGELYDLDASSLQLKVLQ YLQQETRASRCCLLLVSEDNLQLSCKVIGDKVLGEEVSFPLTGCLGQVVE DKKSIQLKDLTSEDVQQLQSMLGCELQAMLCVPVISRATDQVVALACAFN KLEGDLFTDEDEHVIQHCFHYTSTVLTSTLAFQKEQKLKCECQALLQVAK NLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVD DESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRN ILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHS LLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFA SFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYR DPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDIEIFALFISCMCHDLDHR GTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSR KDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLC LLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDRE KAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHK FTIRGLPSNNSLDFLDEEYEVPDLDGTRAPINGCCSLDAE -
Molecular weight information
106 kDa by SDS-PAGE -
Amino acids
2 to 941 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
GenBank Accession No. NM_002599
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
80 µg/ml DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Active human PDE2A full length protein (ab198457)
Specific activity of ab198457 was ≥ 7577 pmol/min/μg.
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SDS-PAGE - Active human PDE2A full length protein (ab198457)SDS-PAGE analysis of ab198457.
Lane 1: 6 µg ab198457
Lane 2: Protein marker