Recombinant human PDE1C/PDE1 protein (ab198450)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human PDE1C/PDE1 protein
See all PDE1C/PDE1 proteins and peptides -
Biological activity
Specific Activity: ≥ 15,000 pmol/min/µg.
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assayed using a PDE1C Assay Kit. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198450 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
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Purity
>= 80 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ESPTKEIEEFESNSLKYLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQL ERGEASVVDLKKNLEYAATVLESVYIDETRRLLDTEDELSDIQSDAVPSE VRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFVERMYRRTSNMV GLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISR FKIPISALVSFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVAN WLTELEIFAIIFSAAIHDYEHTGTTNNFHIQTRSDPAILYNDRSVLENHH LSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIKA MKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQ GDREAELGLPFSPLCDRKSTMVAQSQVGFIDFIVEPTFTVLTDMTEKIVS PLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEGSAPINNSVISV DYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARLAAEEQQKEME AKSQAEEGASGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQ QQNGDFKDGKNKTDKKDHSNIGNDSKKTDDSQE -
Predicted molecular weight
99 kDa including tags -
Amino acids
2 to 634 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_005020.
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Preparation and Storage
- 5''-cyclic nucleotide phosphodiesterase 1C
- Calcium/calmodulin dependent 3'5' cyclic nucleotide phosphodiesterase 1C
- Calcium/calmodulin-dependent 3''
Images
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SDS-PAGE - Recombinant human PDE1C/PDE1 protein (ab198450)
10% SDS-PAGE analysis of 4.41 μg ab198450 with Coomassie staining.
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Functional Studies - Recombinant human PDE1C/PDE1 protein (ab198450)Activity assay using ab198450.
