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Recombinant Human PAP protein (His tag) (ab276344)

Recombinant Human PAP protein (His tag) (ab276344)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 97% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant Human PAP protein (His tag)
    See all PAP proteins and peptides
  • Purity

    > 97 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells
  • Accession

    P15309-2
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MRAAPLLLAR AASLSLGFLF LLFFWLDRSV LAKELKFVTL VFRHGDRSPI DTFPTDPIKE SSWPQGFGQL TQLGMEQHYE LGEYIRKRYR KFLNESYKHE QVYIRSTDVD RTLMSAMTNL AALFPPEGVS IWNPILLWQP IPVHTVPLSE DQLLYLPFRN CPRFQELESE TLKSEEFQKR LHPYKDFIAT LGKLSGLHGQ DLFGIWSKVY DPLYCESVHN FTLPSWATED TMTKLRELSE LSLLSLYGIH KQKEKSRLQG GVLVNEILNH MKRATQIPSY KKLIMYSAHD TTVSGLQMAL DVYNGLLPPY ASCHLTELYF EKGEYFVEMY YRNETQHEPY PLMLPGCSPS CPLERFAELV GPVIPQDWST ECMTTNSHQV LK
    • Predicted molecular weight

      42 kDa including tags
    • Molecular weight information

      The recombinant human ACPP consists of 361 amino acids and has a predicted molecular mass of 42 kDa. The apparent molecular mass of rh ACPP is approximately 45-55 kDa in SDS-PAGE under reducing conditions.
    • Amino acids

      1 to 382
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Predicted N-terminus: Lys 33.
  • Specifications

    Our Abpromise guarantee covers the use of ab276344 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 100% PBS

    • Reconstitution
      This information is lot specific. Please contact our technical Support team for details.

    General Info

    • Alternative names

      • 5' NT
      • 5' nucleotidase
      • 5'-NT
      • 5'-nucleotidase
      • Acid phosphatase prostate
      • ACP 3
      • ACP3
      • acpP
      • Ecto 5' nucleotidase
      • Ecto-5'-nucleotidase
      • PAP
      • PPAP_HUMAN
      • Prostatic acid phosphatase
      • Prostatic acid phosphotase
      • Thiamine monophosphatase
      • TMPase
      see all
    • Sequence similarities

      Belongs to the histidine acid phosphatase family.
    • Cellular localization

      Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
    • Target information above from: UniProt accession P15309 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

Images

  • SDS-PAGE - Recombinant Human PAP protein (His tag) (ab276344)
    SDS-PAGE - Recombinant Human PAP protein (His tag) (ab276344)
    SDS-PAGE analysis of ab276344

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab276344? Please let us know so that we can cite the reference in this datasheet.

    ab276344 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • 5' NT
    • 5' nucleotidase
    • 5'-NT

    Images

    • SDS-PAGE - Recombinant Human PAP protein (His tag) (ab276344)
      SDS-PAGE - Recombinant Human PAP protein (His tag) (ab276344)
      SDS-PAGE analysis of ab276344

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Clear all

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