Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human LMCD1 protein (ab185398)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus, His tag N-Terminus
  • Suitable for: HPLC, SDS-PAGE

Description

  • Product name

    Recombinant Human LMCD1 protein

  • Purity

    > 95 % SDS-PAGE.
    Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    Q9NZU5

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMAKVAKDLNPGVKKMSLGQLQSARGVACLG CKGTCSGFEPHSWRKICKSCKCSQEDHCLTSDLEDDRKIGRLLMDSKYST LTARVKGGDGIRIYKRNRMIMTNPIATGKDPTFDTITYEWAPPGVTQKLG LQYMELIPKEKQPVTGTEGAFYRRRQLMHQLPIYDQDPSRCRGLLENELK LMEEFVKQYKSEALGVGEVALPGQGGLPKEEGKQQEKPEGAETTAATTNG SLSDPSKEVEYVCELCKGAAPPDSPVVYSDRAGYNKQWHPTCFVCAKCSE PLVDLIYFWKDGAPWCGRHYCESLRPRCSGCDEIIFAEDYQRVEDLAWHR KHFVCEGCEQLLSGRAYIVTKGQLLCPTCSKSKRSLEHHH HHH

    • Predicted molecular weight

      44 kDa including tags

    • Amino acids

      1 to 365

    • Tags

      His tag C-Terminus , His tag N-Terminus

    • Specifications

    Our Abpromise guarantee covers the use of ab185398 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      HPLC

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 100% PBS

    General Info

    • Alternative names

      • Dyxin
      • LIM and cysteine rich domains 1
      • LIM and cysteine rich domains protein 1
      • LIM and cysteine-rich domains protein 1
      • LMCD1
      • LMCD1_HUMAN
      see all

    • Function

      Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway.

    • Tissue specificity

      Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.

    • Sequence similarities

      Contains 2 LIM zinc-binding domains.
      Contains 1 PET domain.

    • Domain

      The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.

    • Cellular localization

      Cytoplasm. Nucleus. May shuttle between the cytoplasm and the nucleus.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab185398? Please let us know so that we can cite the reference in this datasheet.

ab185398 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: 100% PBS

Images

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