Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human LIF protein (Animal Free) (ab188052)

Key features and details

  • Expression system: Escherichia coli
  • Purity: >= 98% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human LIF protein (Animal Free)
    See all LIF proteins and peptides

  • Biological activity

    The activity is determined by its ability to induce proliferation of TF-1 cells and is typically less than 0.07 ng/mL.

  • Purity

    >= 98 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P15018

  • Protein length

    Full length protein

  • Animal free

    Yes

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      SPLPITPVNATCAIRHPCHN NLMNQIRSQLAQLNGSANAL FILYYTAQGEPFPNNLDKLC GPNVTDFPPFHANGTEKAKL VELYRIVVYLGTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVLCRLCSKYHVG HVDVTYGPDTSGKDVFQKKK LGCQLLGKYKQIIAVLAQAF

    • Predicted molecular weight

      20 kDa

    • Amino acids

      23 to 202

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab188052 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        SDS-PAGE

        Functional Studies

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Store at -20°C long term. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM acetic acid (AcOH) at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20°C.

      General Info

      • Alternative names

        • CDF
        • Cholinergic Differentiation Factor
        • D factor
        • DIA
        • Differentiation inducing factor
        • differentiation inhibitory activity
        • Differentiation stimulating factor
        • Differentiation-stimulating factor
        • Emfilermin
        • Hepatocyte stimulating factor III
        • HILDA
        • Human interleukin in DA cells
        • Leukemia inhibitory factor
        • LIF
        • LIF_HUMAN
        • Melanoma derived LPL inhibitor
        • Melanoma-derived LPL inhibitor
        • MLPLI
        see all

      • Function

        LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

      • Sequence similarities

        Belongs to the LIF/OSM family.

      • Cellular localization

        Secreted.
      • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab188052? Please let us know so that we can cite the reference in this datasheet.

    ab188052 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • CDF
      • Cholinergic Differentiation Factor
      • D factor
      • DIA
      • Differentiation inducing factor
      • differentiation inhibitory activity
      • Differentiation stimulating factor
      • Differentiation-stimulating factor
      • Emfilermin
      • Hepatocyte stimulating factor III
      • HILDA
      • Human interleukin in DA cells
      • Leukemia inhibitory factor
      • LIF
      • LIF_HUMAN
      • Melanoma derived LPL inhibitor
      • Melanoma-derived LPL inhibitor
      • MLPLI
      see all

    • Function

      LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

    • Sequence similarities

      Belongs to the LIF/OSM family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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