Recombinant human LIF protein (Animal Free) (ab188052)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human LIF protein (Animal Free)
See all LIF proteins and peptides -
Biological activity
The activity is determined by its ability to induce proliferation of TF-1 cells and is typically less than 0.07 ng/mL.
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Purity
>= 98 % SDS-PAGE. -
Endotoxin level
Expression system
Escherichia coliAccession
Protein length
Full length proteinAnimal free
YesNature
Recombinant-
Species
Human -
Sequence
SPLPITPVNATCAIRHPCHN NLMNQIRSQLAQLNGSANAL FILYYTAQGEPFPNNLDKLC GPNVTDFPPFHANGTEKAKL VELYRIVVYLGTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVLCRLCSKYHVG HVDVTYGPDTSGKDVFQKKK LGCQLLGKYKQIIAVLAQAF -
Predicted molecular weight
20 kDa -
Amino acids
23 to 202
Associated products
Specifications
Our Abpromise guarantee covers the use of ab188052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C long term. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 10 mM acetic acid (AcOH) at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20°C.
General Info
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Alternative names
- CDF
- Cholinergic Differentiation Factor
- D factor
see all -
Function
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. -
Sequence similarities
Belongs to the LIF/OSM family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab188052 has not yet been referenced specifically in any publications.
Preparation and Storage
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Alternative names
- CDF
- Cholinergic Differentiation Factor
- D factor
see all -
Function
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. -
Sequence similarities
Belongs to the LIF/OSM family. -
Cellular localization
Secreted. - Information by UniProt
