Recombinant human LDHA protein (Active) (ab93699)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
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Product name
Recombinant human LDHA protein (Active)
See all LDHA proteins and peptides -
Biological activity
Specific activity is > 300 units/mg. in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37°C.
Activity Assay
- Prepare a 1.5 ml reaction mix into a suitable container, adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100mM Sodium phosphate, 0.12 mM beta-nicotinamide adenine dinucleotide, reduced form, 2.3mM Pyruvate, 0.033% (w/v) bovine serum albumin.
- Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer.
- Add 50ul of recombinant LDHA protein in various concentrations (0.1ug, 0.5ug) in assay buffer.
- Mix by inversion and record the increase at A340nm for 5 minutes.
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Purity
> 95 % SDS-PAGE.
ab93699 is purified by using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF -
Predicted molecular weight
39 kDa -
Amino acids
1 to 332 -
Tags
His tag N-Terminus -
Additional sequence information
(NP_005557)
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.316% Tris HCl, 20% Glycerol, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human LDHA protein (His tag) (ab93699)
3ug of ab93699 on 15% SDS-PAGE, stained with Coomassie Blue.
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Western blot - Recombinant human LDHA protein (His tag) (ab93699)
