Recombinant human KDM4D / JMJD2D protein (ab196398)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human KDM4D / JMJD2D protein -
Biological activity
Specific Activity: 0.017 pmole/min/μg
Assay Conditions: 10µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and ab196398 (100 – 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection. -
Purity
> 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ETMKSKANCAQNPNCNIMIFHPTKEEFNDFDKYIAYMESQGAHRAGLAKI IPPKEWKARETYDNISEILIATPLQQVASGRAGVFTQYHKKKKAMTVGEY RHLANSKKYQTPPHQNFEDLERKYWKNRIYNSPIYGADISGSLFDENTKQ WNLGHLGTIQDLLEKECGVVIEGVNTPYLYFGMWKTTFAWHTEDMDLYSI NYLHLGEPKTWYVVPPEHGQRLERLARELFPGSSRGCGAFLRHKVALISP TVLKENGIPFNRITQEAGEFMVTFPYGYHAGFNHGFNCAEAINFATPRWI DYGKMASQCSCGEARVTFSMDAFVRILQPERYDLWKRGQDRAVVDHMEPR VPA -
Predicted molecular weight
67 kDa including tags -
Amino acids
2 to 354 -
Tags
GST tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.12% Glutathione, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human KDM4D / JMJD2D protein (ab196398)
Example of specific activity of ab196398.
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SDS-PAGE - Recombinant human KDM4D / JMJD2D protein (ab196398)4-20% SDS-Page analysis of ab196398 (2.5 μg) with Coomassie staining.