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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Sumo

Anti-p53 BP3 antibody (ab86383)

Anti-p53 BP3 antibody (ab86383)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to p53 BP3
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-p53 BP3 antibody
    See all p53 BP3 primary antibodies
  • Description

    Rabbit polyclonal to p53 BP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residue 995 and 1045 of Human p53 BP3 (NP_005793.2).

  • Positive control

    • HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Sumo
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • Other E3 Ligases
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin like Modifiers

Images

  • Western blot - Anti-p53 BP3 antibody (ab86383)
    Western blot - Anti-p53 BP3 antibody (ab86383)
    All lanes : Anti-p53 BP3 antibody (ab86383) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 119 kDa
    Observed band size: 171 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 BP3 antibody (ab86383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 BP3 antibody (ab86383)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling p53 BPS with ab86383 at 1/200 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-p53 BP3 antibody (ab86383)
    Immunoprecipitation - Anti-p53 BP3 antibody (ab86383)
    Detection of Human p53 BP3 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86383 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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