Recombinant human JAK2 protein (ab42619)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, Inhibition Assay
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Product name
Recombinant human JAK2 protein
See all JAK2 proteins and peptides -
Biological activity
Activity: > 124 pmol/min/ug and is lot specific. If you would like more information about the activity of a specific lot, please contact our scientific support team who will be happy to help.
One unit defined as the amount of enzyme that will transfer 1nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
43 kDa -
Amino acids
808 to 1132 -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human JAK2 protein (ab42619)
Lane 1: 2μg ab42619
Lane 2: Protein marker
Observed band: 43 kDa
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Functional Studies - Recombinant human JAK2 protein (ab42619)Assay was done in Kinase buffer containing 1 mM DTT using Poly-(Glu4:Tyr) substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30oC for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent.
