Recombinant human JAK2 protein (ab42621)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, Inhibition Assay
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Product name
Recombinant human JAK2 protein
See all JAK2 proteins and peptides -
Biological activity
Specific Activity : 8 pmol/min/μg. One unit defined as the amount of enzyme that will transfer 1nmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.
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Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MHHHHHHGGGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKW RNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAE ISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKT YLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDK YLKSSKYIAWPLQGWQATFGGGDHPPKSDPLVPRGSPGEFMNQMVFHKIR NEDLIFNESLGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYS ESFFEAASMMSKLSHKHLVLNYGVCVCGDENILVQEFVKFGSLDTYLKKN KNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGN PPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGT TLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYE PDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDP TQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEH LRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYL QKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVK IGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFG VVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRP DGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG -
Amino acids
532 to 1132 -
Tags
His tag N-Terminus , GST tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human JAK2 protein (ab42621)
Lane 1: 1.4μg ab42621
Lane 2: Protien Marker
Observed weight: 98 kDa
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Functional Studies - Recombinant human JAK2 protein (ab42621)Enzyme reaction is conducted in a buffer containing 50 mM HEPES, pH 7.5, 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, 2 μM substrate (Tyr Peptide 6, Z-lyte kinase assay kit from Invitrogen), and 0-100 ng enzyme at 37°C for 1 hour.
