Recombinant human JAK1 (mutated R724H) protein (Active) (ab268689)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human JAK1 (mutated R724H) protein (Active)
See all JAK1 proteins and peptides -
Biological activity
The specific activity of ab268689 was 28 nmol/min/mg in a kinase assay using synthetic IRS-1 (Y608) peptide (KKHTDDGYMPMSPGVA) as substrate.
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Purity
> 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
NGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQ VQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNIS FMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGE HLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFE AASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLT TPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKL SDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEIC YNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPF FRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFG KVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENI VKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQI CKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTV KDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMAL FLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPS NRTSFQNLIEGFEALLK -
Predicted molecular weight
133 kDa including tags -
Actual molecular weight
28 kDa including tags -
Amino acids
438 to 1154 -
Modifications
mutated R724H -
Tags
GST tag N-Terminus -
Additional sequence information
Genbank: NM_002227
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human JAK1 (mutated R724H) protein (Active) (ab268689)
SDS-PAGE analysis of ab268689.
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Functional Studies - Recombinant human JAK1 (mutated R724H) protein (Active) (ab268689)The specific activity of ab268689 was 28 nmol/min/mg in a kinase assay using synthetic IRS-1 (Y608) peptide (KKHTDDGYMPMSPGVA) as substrate.