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Anti-Rap1 antibody [1D2-1C64] (ab175329)

Key features and details

  • Mouse monoclonal [1D2-1C64] to Rap1
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Rap1 antibody [1D2-1C64]

  • Description

    Mouse monoclonal [1D2-1C64] to Rap1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human Rap1 aa 1-184.
    Sequence:

    MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQ CMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQI LRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKS KINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL


    Database link: P62834
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa, Hek293T, U2OS and mouse NIH 3T3 cell lysates, HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Rap1 antibody [1D2-1C64] (ab175329)
    Western blot - Anti-Rap1 antibody [1D2-1C64] (ab175329)
    All lanes : Anti-Rap1 antibody [1D2-1C64] (ab175329) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HEK293T cell lysate
    Lane 3 : U2OS cell lysate
    Lane 4 : Mouse NIH 3T3 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-mouse IgG-HRP at 1/15000 dilution

    Developed using the ECL technique.

    Predicted band size: 21 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Rap1 antibody [1D2-1C64] (ab175329)
    Immunocytochemistry/ Immunofluorescence - Anti-Rap1 antibody [1D2-1C64] (ab175329)

    Immunofluorescence analysis of formalin-fixed permeabilized HeLa cells, labeling Rap1 (green, left panel) using ab175329 at a 1/100 dilution followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye (central panel).

  • Immunoprecipitation - Anti-Rap1 antibody [1D2-1C64] (ab175329)
    Immunoprecipitation - Anti-Rap1 antibody [1D2-1C64] (ab175329)

    Western blot analysis on immunoprecipitation pellet from mouse NIH 3T3 cells. The antigen-antibody complex was formed by incubating 750 µg of NIH 3T3 whole cell lysate with 2 µg of ab175329 overnight at 4°C. The immune-complex was then captured on 50 µl Protein A/G Plus Agarose, washes extensively and eluted in sample buffer. 1) 25 µg of NIH 3T3 whole cell lysate, as a control, and 2) eluted sample were resolved on a SDS PAGE gel. The membrane was probed with ab175329 at a 1/500 dilution. Chemiluminescent detection was perfomed.

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