Recombinant Human IL3RA/CD123 protein (Fc Chimera) (ab88358)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
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Product name
Recombinant Human IL3RA/CD123 protein (Fc Chimera)
See all IL3RA/CD123 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
TKEDPNPPITNLRMKAKAQQLTWDLNRNVTDIECVKDADYSMPAVN NS YCQFGAISLCEVTNYTVRVANPPFSTWILFPENSGKPWAGAENL TCWI HDVDFLSCSWAVGPGAPADVQYDLYLNVANRRQQYECLHYKT DAQGTR IGCRFDDISRLSSGSQSSHILVRGRSAAFGIPCTDKFVVF SQIEILTP PNMTAKCNKTHSFMHWKMRSHFNRKFRYELQIQKRMQP VITEQVRDRT SFQLLNPGTYTVQIRARERVYEFLSAWSTPQRFECD QEEGANTRGGRV DGIQWIPKVDKKVEPKSCDKTHTCPPCPAPELLG GPSVFLFPPKPKDT LMISRTPEVTCVVVDVSHEDPEVKFNWYVDGV EVHNAKTKPREEQYNS TYRVVSVLTVLHQDWLNGKEYKCRVSNKAL PAPIEKTISKAKGQPREP QVYTLPPSRDELTKNQVSLTCLVKGFYP SDIAVEWESNGQPENNYKTT PPVLDSDGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKSLS LSPGK -
Amino acids
19 to 302 -
Additional sequence information
DNA sequence encoding the signal peptide and extracellular domain of human IL3R alpha (aa 1-18) was fused to the Fc region of human IgG1 (aa 93-330). Protein expressed in modified human 293 cells.
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Preparation and Storage
- CD123
- CD123 antigen
- hIL 3Ra
Images
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SDS-PAGE - Recombinant Human IL3RA/CD123 protein (Fc Chimera) (ab88358)1D SDS-PAGE of ab88358 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab88358
Lane 2: ab88358 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab88358 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Drop in MWt after treatment with PNGase F indicates presence of N-linked glycans. Subsequent drop in MWt after treatment with glycosidase cocktail indicates presence of O-linked glycans. Faint bands in lane 2 and lane 3 are glycosidase enzymes. -
SDS-PAGE - Recombinant Human IL3RA/CD123 protein (Fc Chimera) (ab88358)A sample of ab88358 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot train indicates presence of multiple isoforms of ab88358. -
Functional Studies - Recombinant Human IL3RA/CD123 protein (Fc Chimera) (ab88358)Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MWt and pI of the protein.
