Recombinant human IL-4 protein (ab83686)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human IL-4 protein
See all IL-4 proteins and peptides -
Biological activity
Activity: The ED50 of ab83686 is typically 0.02 - 0.2 ng/ml as measured in a cell proliferation assay using the human growth factor-dependent TF1 cell line. -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
Theoretical Sequence: HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNT TEKETFCRAATVLRQFYSHHEKDTRCL GATAQQFHRHKQLIRFLKRLD RNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: PBS, 1% Human serum albumin, 10% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Images
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Functional Studies - Recombinant human IL-4 protein (ab83686)Densitometry of protein isoforms visualised by 2-DE. The triangle indicates the theoretical MW and pI of the protein.
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SDS-PAGE - Recombinant human IL-4 protein (ab83686)1D SDS-PAGE of ab83686 before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation.
Lane 1: ab83686
Lane 2: ab83686 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83686 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 μg protein loaded per lane; Deep Purple™ stained.
Additional bands in lane 2 and lane 3 are glycosidase enzymes.
