Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NFIL3 antibody (ab88526)

Key features and details

  • Rabbit polyclonal to NFIL3
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-NFIL3 antibody
    See all NFIL3 primary antibodies

  • Description

    Rabbit polyclonal to NFIL3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human NFIL3 aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab103932)

  • Positive control

    • This antibody gave a positive signal in the following Human whole cell lysates: HeLa; K562.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab88526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 mg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
Notes
WB
Use a concentration of 1 mg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).

Target

  • Function

    Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death.

  • Tissue specificity

    Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.

  • Sequence similarities

    Belongs to the bZIP family. NFIL3 subfamily.
    Contains 1 bZIP domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • E4 promoter binding protein 4 antibody
      • E4 promoter-binding protein 4 antibody
      • E4BP 4 antibody
      • E4BP4 antibody
      • E4BP4 protein antibody
      • IL3BP 1 antibody
      • IL3BP1 antibody
      • Interleukin 3 binding protein 1 antibody
      • Interleukin 3 promoter transcriptional activator antibody
      • Interleukin-3 promoter transcriptional activator antibody
      • Interleukin-3-binding protein 1 antibody
      • NFIL 3 antibody
      • NFIL 3A antibody
      • NFIL3 antibody
      • NFIL3_HUMAN antibody
      • NFIL3A antibody
      • Nuclear factor interleukin 3 regulated antibody
      • Nuclear factor interleukin-3-regulated protein antibody
      • Transcriptional activator NF IL3A antibody
      • Transcriptional activator NF-IL3A antibody
      see all

    Images

    • Western blot - Anti-NFIL3 antibody (ab88526)
      Western blot - Anti-NFIL3 antibody (ab88526)
      All lanes : Anti-NFIL3 antibody (ab88526) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 51 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      Abcam has observed other anti-NFIL3 antibodies react with a 55 kDa band. Abcam welcomes customer feedback.

    References (0)

    Publishing research using ab88526? Please let us know so that we can cite the reference in this datasheet.

    ab88526 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-NFIL3 antibody (ab88526)
      Western blot - Anti-NFIL3 antibody (ab88526)
      All lanes : Anti-NFIL3 antibody (ab88526) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 51 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      Abcam has observed other anti-NFIL3 antibodies react with a 55 kDa band. Abcam welcomes customer feedback.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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