Human IL-1R3 ELISA Kit (ab267659)
Key features and details
- Sensitivity: 18 pg/ml
- Range: 16.38 pg/ml - 4000 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-1R3 ELISA Kit
See all IL-1RAcP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
18 pg/ml -
Range
16.38 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 106 91% - 129% Plasma 113 102% - 132% Cell culture media 105 92% - 111% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-1R3 ELISA Kit is designed for the quantitative determination of IL-1R3 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human IL-1R3 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1R3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-1R3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1R3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human IL-1R3 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human IL-1R3 Detection Antibody 2 vials Human IL-1R3 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. -
Tissue specificity
Detected in liver, skin, placenta, thymus and lung. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- C3orf13
- FLJ37788
- IL 1 receptor accessory protein
see all -
Database links
- Entrez Gene: 3556 Human
- Omim: 602626 Human
- SwissProt: Q9NPH3 Human
- Unigene: 478673 Human
Images
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Standard curve
Human IL-1R3 ELISA kit (ab267659) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.