Recombinant human IL-7 protein (Active) (ab259382)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, MS, HPLC, Cell Culture
Preparation and Storage
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Alternative names
- IL 7
- IL-7
- Il7
see all -
Function
Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. -
Sequence similarities
Belongs to the IL-7/IL-9 family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human IL-7 protein (Active) (ab259382)
Fully biologically active determined by the dose dependant proliferation of 2E8 cells.
ED50 is ≤ 2 ng/mL, corresponding to a specific activity of 4.55 x 105 units/mg.
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SDS-PAGE - Recombinant human IL-7 protein (Active) (ab259382)SDS-PAGE analysis of ab259382.
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HPLC - Recombinant human IL-7 protein (Active) (ab259382)Purity = 98.5%.
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 µm). 5 µL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
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Mass Spectrometry - Recombinant human IL-7 protein (Active) (ab259382)M+1.38 Da (+365 Da: Hex(1)HexNAc(1); +203 Da: HexNAc: +283 Da: s/pGlcNac) (Calc. mass 17444.62 Da).
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 µm, Thermo Scientific). 5 µL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).