Recombinant human IgG4 protein (Active) (ab181968)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.4
Constituents: 0.41% Tris, 0.51% Glycine, 6.8% Trehalose, 0.04% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 500 µg/ml.
Images
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SDS-PAGE - Recombinant Human IgG4 protein (ab181968)
SDS-PAGE of DDT-reduced (lane 1) and non-reduced (lane 2) ab181968 stained overnight with Coomassie Blue.
As a result of glycosylation, DTT-reduced (R) protein migrates as 30-32 kDa and non-reduced (NR) protein migrates as 28-30 and 55-60 kDa.
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Surface Plasmon Resonance - Recombinant human IgG4 protein (Active) (ab181968)Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) captured on CM5 Chip via anti-His antibody can bind ab181968 with an affinity constant of 0.715 μM as determined in SPR assay (Biacore).