Recombinant human IDH2 protein (ab198092)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 59% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human IDH2 protein
See all IDH2 proteins and peptides -
Biological activity
Specific Activity: 14,200 pmol/min/µg.
Assay Conditions:
The reaction mixture contained 50 mM Tris, pH 7.4, 5 mM MgCl2, 200 µM DL-isocitrate, 200 µM NADP+, and various amount of ab198092 in a volume of 200 µL. Reactions were initiated with the addition of ab198092, and the increase in NADPH absorbance at 340 nm was continuously monitored for 7 min. Molar extinction coefficient of NADPH is 6,220 M-1cm-1.
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Purity
>= 59 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MAGYLRVVRSLCRASGSRPAWAPAALTAPTSQEQPRRHYADKRIKVAKPV VEMDGDEMTRIIWQFIKEKLILPHVDIQLKYFDLGLPNRDQTDDQVTIDS ALATQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFRE PIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKD GSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMST KNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVAQVLKS SGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGT VTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEK VCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALG RQDYKDDDDK -
Predicted molecular weight
52 kDa including tags -
Amino acids
1 to 452 -
Tags
DDDDK tag C-Terminus -
Additional sequence information
NM_002168.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Contains 90 µg/ml DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human IDH2 protein (ab198092)
Activity assay of ab198092.
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SDS-PAGE - Recombinant human IDH2 protein (ab198092)10% SDS-PAGE analysis of 1.4 µg ab198092 with Coomassie staining.
