Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UAP1 antibody [EPR10259] (ab155287)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10259] to UAP1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-UAP1 antibody [EPR10259]
    See all UAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10259] to UAP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A synthetic peptide, corresponding to residues in Human UAP1 (UniProt: Q16222).

  • Positive control

    • Human testis, Jurkat and A549 lysates; Human gastric carcinoma tissue; Permeabilized Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-UAP1 antibody [EPR10259] (ab155287)
    Western blot - Anti-UAP1 antibody [EPR10259] (ab155287)
    All lanes : Anti-UAP1 antibody [EPR10259] (ab155287) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 59 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-UAP1 antibody [EPR10259] (ab155287)
    Immunocytochemistry/ Immunofluorescence - Anti-UAP1 antibody [EPR10259] (ab155287)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling UAP1 with purified ab155287 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling UAP1 with ab155287 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-UAP1 antibody [EPR10259] (ab155287)
    Flow Cytometry - Anti-UAP1 antibody [EPR10259] (ab155287)
    Flow cytometric analysis of permeabilized Jurkat cells labeling UAP1 with ab155287 at 1/100 dilution (red) compared with a rabbit IgG negative control (green).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)

    Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab155287 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)

    Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab155287 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)

    Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155287 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)

    Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab155287 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-UAP1 antibody [EPR10259] (ab155287)
    Anti-UAP1 antibody [EPR10259] (ab155287)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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