Anti-UAP1 antibody [EPR10259] (ab155287)
![Anti-UAP1 antibody [EPR10259] (ab155287)](https://www.abcam.com/ps/products/155/ab155287/Images/ab155287-162379-anti-uap1-antibody-epr10259-western-blot.jpg "Anti-UAP1 antibody [EPR10259] (ab155287)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10259] to UAP1
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-UAP1 antibody [EPR10259]
See all UAP1 primary antibodies -
Description
Rabbit monoclonal [EPR10259] to UAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
A synthetic peptide, corresponding to residues in Human UAP1 (UniProt: Q16222).
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Positive control
- Human testis, Jurkat and A549 lysates; Human gastric carcinoma tissue; Permeabilized Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10259 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-UAP1 antibody [EPR10259] (ab155287)All lanes : Anti-UAP1 antibody [EPR10259] (ab155287) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 59 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-UAP1 antibody [EPR10259] (ab155287)](https://www.abcam.com/ps/products/155/ab155287/Images/ab155287-265177-anti-uap1-antibody-epr10259-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-UAP1 antibody [EPR10259] (ab155287)Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling UAP1 with purified ab155287 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)](https://www.abcam.com/ps/products/155/ab155287/Images/ab155287-162378-anti-uap1-antibody-epr10259-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling UAP1 with ab155287 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-UAP1 antibody [EPR10259] (ab155287)](https://www.abcam.com/ps/products/155/ab155287/Images/ab155287-162377-anti-uap1-antibody-epr10259-flow-cytometry.jpg)
Flow Cytometry - Anti-UAP1 antibody [EPR10259] (ab155287)Flow cytometric analysis of permeabilized Jurkat cells labeling UAP1 with ab155287 at 1/100 dilution (red) compared with a rabbit IgG negative control (green). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)](https://www.abcam.com/ps/products/155/ab155287/Images/ab155287-162376-anti-uap1-antibody-epr10259-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab155287 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)](https://www.abcam.com/ps/products/155/ab155287/Images/ab155287-162375-anti-uap1-antibody-epr10259-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab155287 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)](https://www.abcam.com/ps/products/155/ab155287/Images/ab155287-162374-anti-uap1-antibody-epr10259-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155287 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)](https://www.abcam.com/ps/products/155/ab155287/Images/ab155287-162373-anti-uap1-antibody-epr10259-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UAP1 antibody [EPR10259] (ab155287)Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab155287 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-UAP1 antibody [EPR10259] (ab155287)](https://www.abcam.com/ps/products/155/ab155287/Images/ab155287-9-benefits-of-recombinant-antibodies.png)
Anti-UAP1 antibody [EPR10259] (ab155287)
