Recombinant human IDH1 protein (ab198096)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human IDH1 protein
See all IDH1 proteins and peptides -
Biological activity
Specific Activity: 5600 pmol/min/µg.
Assay Conditions: Reaction was conducted in 50 mM Tris (pH 7.4), 10 mM MgCl2, 200 µM DL-isocitrate, and 200 µM NADP. Incubate for 30 minutes at room temperature. Read at Abs340.
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Purity
> 80 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRD ATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIR NILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPG KVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMA LSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRL IDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDG KTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNK ELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDK LGENLKIKLAQAKL -
Predicted molecular weight
48 kDa including tags -
Amino acids
1 to 414 -
Tags
DDDDK tag C-Terminus -
Additional sequence information
GenBank Accession No. NM_005896.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 10% Glycerol (glycerin, glycerine)
Contains 90 µg/ml DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human IDH1 protein (ab198096)
Activity assay using ab198096.
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SDS-PAGE - Recombinant human IDH1 protein (ab198096)10% SDS-PAGE analysis of 2.5 µg ab198096 with Coomassie staining.
