Recombinant human HtrA3 protein (ab134450)
Key features and details
- Expression system: Insect cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Inhibition Assay, Functional Studies
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Product name
Recombinant human HtrA3 protein
See all HtrA3 proteins and peptides -
Biological activity
Proteolytic activity of ab134450 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 50 µg/ml HtrA3 within 2 hours at 37°C. -
Purity
> 70 % SDS-PAGE. -
Expression system
Insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
48 kDa including tags -
Amino acids
1 to 453 -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 50% GlycerolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human HtrA3 protein (ab134450)SDS-PAGE analysis of ab134450 (1µg). Minor bands of HtrA3 fragments may be visible in the enzyme preparation.
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SDS-PAGE - Recombinant human HtrA3 protein (ab134450)Hydrolysis of beta-Casein by ab134450. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5 mM CaCl2 with or without HtrA3 (50 µg/ml). After indicated time intervals 10 µl aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.
