Recombinant human htrA1 protein (ab134441)
Key features and details
- Expression system: Insect cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human htrA1 protein
See all htrA1 proteins and peptides -
Biological activity
Proteolytic activity of ab134441 was documented by digestion of ß- casein. 0.5 mg ß-casein/ml are completely digested by 5 µg/ml ab134441 within 3 hours at 37°C. -
Purity
> 70 % SDS-PAGE.
ab134441 was purified from insect cell culture supernatants. -
Expression system
Insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
50 kDa including tags -
Amino acids
1 to 480 -
Tags
His tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Preservative: 0.34% Imidazole
Constituents: 0.05% Brij, 0.05% Calcium chloride, 0.79% Tris HCl, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human htrA1 protein (ab134441)SDS-PAGE analysis of ab134441 (2µg).
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SDS-PAGE - Recombinant human htrA1 protein (ab134441)Hydrolysis of ß-Casein by ab134441. ß-Casein (0.5 mg/ml) was incubated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2 without or with ab134441 (5 µg/ml). After various time intervals aliquots of the reaction mixtures were withdrawn and analyzed by SDS-PAGE.
