Recombinant Human HSF1 protein (ab204184)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE
-
Product name
Recombinant Human HSF1 protein
See all HSF1 proteins and peptides -
Purity
> 90 % SDS-PAGE.
Constructed with C-terminal 11 arginine tag, which efficiently delivers the protein intracellularly. ab204184 was expressed in E.coli as inclusion bodies, refolded using a unique temperature shift inclusion body refolding technology and chromatographically purified. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MASMTGGQQMGRGHHHHHHENLYFQGGEFDLPVGPGAAGPSNVPAFLTKL WTLVSDPDTDALICWSPSGNSFHVFDQGQFAKEVLPKYFKHNNMASFVRQ LNMYGFRKVVHIEQGGLVKPERDDTEFQHPCFLRGQEQLLENIKRKVTSV STLKSEDIKIRQDSVTKLLTDVQLMKGKQECMDSKLLAMKHENEALWREV ASLRQKHAQQQKVVNKLIQFLISLVQSNRILGVKRKIPLMLNDSGSAHSM PKYSRQFSLEHVHGSGPYSAPSPAYSSSSLYAPDAVASSGPIISDITELA PASPMASPGGSIDERPLSSSPLVRVKEEPPSPPQSPRVEEASPGRPSSVD TLLSPTALIDSILRESEPAPASVTALTDARGHTDTEGRPPSPPPTSTPEK CLSVACLDKNELSDHLDAMDSNLDNLQTMLSSHGFSVDTSALLDLFSPSV TVPDMSLPDLDSSLASIQELLSPQEPPRPPEAENSSPDSGKQLVHYTAQP LFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAEDPTISLLTGSEPPK AKDPTVSLEESGGGGSPGRRRRRRRRRRR -
Predicted molecular weight
57 kDa -
Amino acids
2 to 529 -
Tags
His-T7 tag N-Terminus -
Additional sequence information
Expressed with a small T7-His-TEV cleavage site Tag (29 amino acids) fusion at the N-terminal and a Polyarginine tag at the C-terminal (NP_005517).
-
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and Glycerol.
