Recombinant human HGF protein (Active) (ab259401)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, HPLC, MS, Functional Studies
Preparation and Storage
- DFNB39
- F TCF
- Fibroblast derived tumor cytotoxic factor
Images
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Functional Studies - Recombinant human HGF protein (Active) (ab259401)
Fully biologically active determined by the dose dependent induce IL-11 secretion by Saos‑2 human osteosarcoma cells.
ED50 is ≤4.9 ng/ml, corresponding to a specific activity of 0.20 x 106 units/mg.
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SDS-PAGE - Recombinant human HGF protein (Active) (ab259401)SDS-PAGE analysis of ab259401.
Additional lower MW bands are result of protein degradation during the production process and do not affect protein function.
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Mass Spectrometry - Recombinant human HGF protein (Active) (ab259401)Mass determination by ESI TOF.
Predicted mass is 79720.38 Da (+/- 10 Da by ESI TOF). Observed mass is 79724.93.
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HPLC - Recombinant human HGF protein (Active) (ab259401)HPLC analysis of ab259401.