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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Recombinant human HDAC5 protein (ab80348)

Recombinant human HDAC5 protein (ab80348)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: Baculovirus
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant human HDAC5 protein
    See all HDAC5 proteins and peptides
  • Biological activity

    Specific Activity: ≥2500 pmol/min/µg.
    Unit Definition: One U =1 pmol of acetyl group removed/min/µg of enzyme.
    Assay Conditions:
    25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM substrate, and 0.8-50 ng/µl HDAC5. Incubation condition: 30 min at 37°C followed by HDAC developer for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.

     

  • Expression system

    Baculovirus
  • Accession

    Q9UQL6
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      1 to 1122
    • Tags

      GST tag N-Terminus

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.395% Tris HCl, 0.05% Tween, 10% Glycerol (glycerin, glycerine), 0.8004% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • Functional Studies - Recombinant human HDAC5 protein (ab80348)
    Functional Studies - Recombinant human HDAC5 protein (ab80348)
    Image showing specific activity of ab80348.
  • SDS-PAGE - Recombinant human HDAC5 protein (ab80348)
    SDS-PAGE - Recombinant human HDAC5 protein (ab80348)
    10% SDS-PAGE showing ab80348 at approximately 150kDa (7µg).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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