Recombinant human HDAC1 protein (ab104121)
Key features and details
- Expression system: Baculovirus
- Purity: > 50% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human HDAC1 protein
See all HDAC1 proteins and peptides -
Biological activity
Specific Activity: 460 pmol/min/µg.
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Purity
> 50 % SDS-PAGE.
Purity is >50% as determined by SDS-PAGE. -
Expression system
Baculovirus -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
56 kDa -
Amino acids
1 to 482 -
Tags
His tag C-Terminus , DDDDK tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.395% Tris HCl, 0.05% Tween, 10% Glycerol (glycerin, glycerine), 0.754% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human HDAC1 protein (ab104121)10% SDS-PAGE showing ab104121 at approximately 56kDa (1µg).
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Functional Studies - Recombinant human HDAC1 protein (ab104121)HDAC1 specific activity. HDAC reaction was carried out for 30 minutes at 37°C in a solution containing 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA, 20 µM Fluorogenic HDAC substrate, and varying amounts of HDAC1 from 0-80 ng. This was followed by treatment with 50 µL of HDAC developer for 15 minutes at room temperature. Fluorescence intensity is measured at ex360/em460.
