Recombinant human FUK protein (ab198052)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human FUK protein -
Biological activity
Specific Activity: ≥85.3 pmole/min/µg
Assay Conditions: The reaction mixture contained 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 2 mM L-Fucose, 100 μM ATP, and various amount of fucokinase in a final volume of 50 μL. The reaction was incubated at 37°C for 1 hr, and the amount of ATP consumed was calculated.
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Purity
> 95 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MDYKDDDDKEQPKGVDWTVIILTCQYKDSVQVFQRELEVRQKREQIPAGT LLLAVEDPEKRVGSGGATLNALLVAAEHLSARAGFTVVTSDVLHSAWILI LHMGRDFPFDDCGRAFTCLPVENPEAPVEALVCNLDCLLDIMTYRLGPGS PPGVWVCSTDMLLSVPANPGISWDSFRGARVIALPGSPAYAQNHGVYLTD PQGLVLDIYYQGTEAEIQRCVRPDGRVPLVSGVVFFSVETAERLLATHVS PPLDACTYLGLDSGARPVQLSLFFDILHCMAENVTREDFLVGRPPELGQG DADVAGYLQSARAQLWRELRDQPLTMAYVSSGSYSYMTSSASEFLLSLTL PGAPGAQIVHSQVEEQQLLAAGSSVVSCLLEGPVQLGPGSVLQHCHLQGP IHIGAGCLVTGLDTAHSKALHGRELRDLVLQGHHTRLHGSPGHAFTLVGR LDSWERQGAGTYLNVPWSEFFKRTGVRAWDLWDPETLPAEYCLPSARLFP VLHPSRELGPQDLLWMLDHQEDGGEALRAWRASWRLSWEQLQPCLDRAAT LASRRDLFFRQALHKARHVLEARQDLSLRPLIWAAVREGCPGPLLATLDQ VAAGAGDPGVAARALACVADVLGCMAEGRGGLRSGPAANPEWMRPFSYLE CGDLAAGVEALAQERDKWLSRPALLVRAARHYEGAGQILIRQAVMSAQHF VSTEQVELPGPGQWVVAECPARVDFSGGWSDTPPLAYELGGAVLGLAVRV DGRRPIGARARRIPEPELWLAVGPRQDEMTVKIVCRCLADLRDYCQPHAP GALLKAAFICAGIVHVHSELQLSEQLLRTFGGGFELHTWSELPHGSGLGT SSILAGTALAALQRAAGRVVGTEALIHAVLHLEQVLTTGGGWQDQVGGLM PGIKVGRSRAQLPLKVEVEEVTVPEGFVQKLNDHLLLVYTGKTRLARNLL QDVLRSWYARLPAVVQNAHSLVRQTEECAEGFRQGSLPLLGQCLTSYWEQ KKLMAPGCEPLTVRRMMDVLAPHVHGQSLAGAGGGGFLYLLTKEPQQKEA LEAVLAKTEGLGNYSIHLVEVDTQGLSLKLLGTEASTCCPFP -
Predicted molecular weight
119 kDa including tags -
Amino acids
2 to 1084 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
GenBank Accession No. NM_145059
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Tween, 0.64% Sodium chloride
Also contaings 80 ng/µL DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human FUK protein (ab198052)
Specific activity of ab198052 was determined to be 85.3 pmol/min/μg
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SDS-PAGE - Recombinant human FUK protein (ab198052)4-20% SDS-PAGE of FUK using 2μg of ab198052.