Recombinant human Flt3 / CD135 protein (Fc Chimera Active) (ab83998)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
-
Product name
Recombinant human Flt3 / CD135 protein (Fc Chimera Active)
See all Flt3 / CD135 proteins and peptides -
Biological activity
Activity: The ED50 of ab83998 is typically 1.0 -2.0 ng/ml as measured by its ability to neutralize Flt3 mediated proliferation of the OCI/AML5 human leukemia cell line. -
Purity
> 95 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
Theoretical Sequence: NQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGC ALRPQSSGTVYEAAAVEVDVSASITLQVL VDAPGNISCLWVFKHSSLN CQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNT L LYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESP AVVKKEEKVLHELFGTDIRCC ARNELGRECTRLFTIDLNQTPQTTLPQ LFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEM STYSTNR TMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSE DYEIDQYEEFCFS VRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISK FCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRK PQVLAEASASQASCF SDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNM SEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDRIPKVDKKVEP KSCDKTHTCPPCPAPELLGGPS VFLFPPKPKDTLMISRTPEVTCVVVD VSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVL TVLHQDW LNGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQV SLTCLVKGFYP SDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLT VDKSRWQQGNVFSCSVMHEALHNHYTQKS LSLSPGK -
Additional sequence information
Fused with the Fc region of Human IgG1 at the C-terminus.
-
Preparation and Storage
-
Alternative names
- CD 135
- CD135
- CD135 antigen
see all -
Function
Receptor for the FL cytokine. Has a tyrosine-protein kinase activity. -
Tissue specificity
Bone marrow cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
Images
-
Functional Studies - Recombinant human Flt3 / CD135 protein (Fc Chimera Active) (ab83998)Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein. -
SDS-PAGE - Recombinant human Flt3 / CD135 protein (Fc Chimera Active) (ab83998)1D SDS-PAGE of ab83998 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83998
Lane 2: ab83998 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83998 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
10 μg protein loaded per lane; Deep Purple™ stained. -
SDS-PAGE - Recombinant human Flt3 / CD135 protein (Fc Chimera Active) (ab83998)A sample of ab83998 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 μg protein loaded. Spot train indicates presence of multiple isoforms.
