Recombinant human FGF21 protein (Active) (ab269248)
Key features and details
- Expression system: Escherichia coli
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE
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Product name
Recombinant human FGF21 protein (Active)
See all FGF21 proteins and peptides -
Biological activity
3T3 proliferation with β-klotho and heparin ED50 ≤ 500 ng/mL (≥ 2.0 x 10^3 unit/mg).
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Purity
>95% by SDS-PAGE . -
Endotoxin level
Expression system
Escherichia coliAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Human -
Sequence
MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELLLEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPALPEP PGILAPQ -
Predicted molecular weight
22 kDa -
Amino acids
29 to 184
Associated products
Specifications
Our Abpromise guarantee covers the use of ab269248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..
Constituents: 0.16% Sodium phosphate, 0.29% Sodium chloride
Lyophilized fromThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionSterile water at 0.1 mg/mL
General Info
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Alternative names
- FGF 21
- FGF-21
- Fgf21
see all -
Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Recombinant human FGF21 protein (Active) (ab269248)SDS-PAGE analysis of ab269248 at 1ug/lane under (-) non-reducing and (+) reducing conditions. 4-20% Tris glycine gel. Stained with coomassie blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab269248 has not yet been referenced specifically in any publications.
Preparation and Storage
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Alternative names
- FGF 21
- FGF-21
- Fgf21
see all -
Function
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. - Information by UniProt
Images
-
SDS-PAGE - Recombinant human FGF21 protein (Active) (ab269248)SDS-PAGE analysis of ab269248 at 1ug/lane under (-) non-reducing and (+) reducing conditions. 4-20% Tris glycine gel. Stained with coomassie blue.