Recombinant human FGF 23 protein (ab108553)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human FGF 23 protein (ab108553)ERK and FRS2aplha phosphorylation induced by FGF 23 in Klotho expressing cells.
Klotho expressing HEK 293EBNA cells were serum starved for 16hr and then stimulated with hFGF 23-His, FGF 23-Fc (ab108553), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 min, respectively.
Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.
Lane 1: Mock (non-treated)
Lane 2: Mock + hFGF 23-Fc (ab108553) 1µg/ml
Lane 3: Mock + hFGF 23-His 1µg/ml
Lane 4: Klotho + hFGF 23-Fc (ab108553) 1µg/ml
Lane 5: Klotho + hFGF 23-Fc (ab108553) 4µg/ml
Lane 6: Klotho + mCD137-Fc 1µg/ml
Lane 7: Klotho (non-treated)
Lane 8: Klotho + 100ng/ml FGF-b
