Antibodies, Proteins, Kits and Reagents for Life Science

Recombinant human Fas protein (ab155635)

Key features and details

Expression system: HEK 293 cells
Purity: > 95% SDS-PAGE
Endotoxin level:
Active: Yes
Suitable for: Functional Studies, SDS-PAGE

Alternative names
- ALPS 1A
- ALPS1A
- APO 1
- Apo 1 antigen
- APO 1 cell surface antigen
- Apo-1 antigen
- APO1
- Apo1 antigen
- APO1 cell surface antigen
- Apoptosis antigen 1
- Apoptosis mediating surface antigen FAS
- Apoptosis-mediating surface antigen FAS
- APT 1
- APT1
- CD 95
- CD 95 antigen
- CD95
- CD95 antigen
- Delta Fas
- Delta Fas/APO 1/CD95
- Delta Fas/APO1/CD95
- Fas
- Fas (TNF receptor superfamily, member 6)
- FAS 1
- FAS 827dupA
- Fas AMA
- FAS Antigen
- Fas cell surface death receptor
- FAS1
- FASLG receptor
- FASTM
- sFAS
- Surface antigen APO1
- TNF receptor superfamily, member 6
- TNFRSF 6
- TNFRSF6
- TNR6_HUMAN
- Tumor necrosis factor receptor superfamily member 6
see all
Function

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Tissue specificity

Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Involvement in disease

Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Sequence similarities

Contains 1 death domain.
Contains 3 TNFR-Cys repeats.
Domain

Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
Cellular localization

Secreted and Cell membrane.
Target information above from: UniProt accession P25445 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

SDS-PAGE - Recombinant human Fas protein (ab155635)

Human Fas, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Functional Studies - Recombinant human Fas protein (ab155635)

Immobilized Human Fas Ligand, His Tag at 2 µg/mL (100 µL/well)can bind Human Fas, Fc Tag with a linear range of 4-31 ng/mL.
SDS-PAGE - Recombinant human Fas protein (ab155635)

SDS PAGE analysis of reduced ab155635 stained overnight with Coomassie Blue.

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