Recombinant human EGFR (mutated T790M) protein (Active) (ab271493)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 72% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human EGFR (mutated T790M) protein (Active)
See all EGFR proteins and peptides -
Biological activity
40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr)-biotin substrate and 20 mM ATP. Reaction was performed at 30°C for 35 mins. Amount of ATP transferred was calculated using Kinase-Glo reagent.
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Purity
>= 72 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKV LGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMA SVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNW CVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEK EYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDG IPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIE FSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYL IPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQ RYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNP APSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISL DNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA -
Predicted molecular weight
88 kDa -
Amino acids
668 to 1210 -
Tags
His tag C-Terminus , GST tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.4% Tris HCl, 0.8% Sodium chloride, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human EGFR (mutated T790M) protein (Active) (ab271493)Specific activity of ab271493.
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SDS-PAGE - Recombinant human EGFR (mutated T790M) protein (Active) (ab271493)SDS-PAGE analysis of 2 µg ab271493.