Recombinant human DR5 protein (Fc Chimera Active) (ab174009)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: ELISA, SDS-PAGE, Functional Studies
Preparation and Storage
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Alternative names
- Fas like protein
- Apoptosis inducing protein TRICK2A/2B
- Apoptosis inducing receptor TRAIL R2
see all -
Function
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. -
Tissue specificity
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. -
Involvement in disease
Squamous cell carcinoma of the head and neck -
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant human DR5 protein (Fc Chimera Active) (ab174009)Human DR5, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant human DR5 protein (Fc Chimera Active) (ab174009)Immobilized recombinant Human TNFSF10 at 5 µg/mL (100 µL/well) can bind Human DR5, Fc Tag with a linear range of 0.4-13 ng/mL. -
SDS-PAGE - Recombinant human DR5 protein (Fc Chimera Active) (ab174009)SDS-PAGE analysis of reduced ab174009 stained overnight with Coomassie Blue.
DTT-reduced Protein migrates as 46 kDa due to glycosylation.
