Nitric Oxide Assay Kit (Flow cytometry - Orange) (ab219933)
Key features and details
- Assay type: Cell-based
- Detection method: Flow cytometry-fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Nitric Oxide Assay Kit (Flow cytometry - Orange)
See all Nitric oxide kits -
Detection method
Flow cytometry-fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Nitric Oxide Assay Kit (Flow cytometry - Orange) (ab219933) is a sensitive fluorometric assay to monitor intracellular nitric oxide (NO) levels in live cells using a flow cytometer. The assay uses an orange dye that can react with NO to generate a bright orange fluorescent product that can be easily detected at Ex/Em = 540/590 nm, using the same filter set as Cy3® or TRITC.
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Notes
Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes. Altered NO production is implicated in various immunological, cardiovascular, neurodegenerative and inflammatory diseases. As a free radical, NO is rapidly oxidized and there is relatively low concentrations of NO existing in vivo. It has been challenging to detect and understand the role of NO in biological systems.
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Platform
Flow cytometer
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 500X Orange Dye 1 x 100µl Assay Buffer 1 x 10ml NONOate Positive Control 1 vial -
Research areas
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Relevance
Nitric oxide (NO) is a key vertebrate biological messenger, playing an important role in neurotransmission, vascular regulation, immune responses and apoptosis. NO , also known as "endothelium-derived relaxing factor" or "EDRF", is synthesized from L-arginine, oxygen and NADPH by various NO synthases. Most of the NO in the cell is oxidized to nitrite (NO2-) and nitrate (NO3-), and therefore the concentrations of these anions are generally as a quantitative measure of NO production.
Images
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Nitric Oxide Assay Kit (Flow Cytometry-Orange) (ab219933)
Exogenous nitric oxide (NO) production in Jurkat cells upon DEA/NONOate treatment (NO donor). Cells were incubated with the NO Orange Dye in a 37°C/5% CO2 incubator for 30 minutes, followed by a wash in HHBS buffer. Cells were then left untreated (blue) or further treated with 1 mM DEA/NONOate (red) in Assay Buffer at 37°C for an additional 30 minutes. Fluorescent intensitites were measured with a FACScalibur flow cytometer (BD Biosciences) using FL2 channel.