Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) (ab271467)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, DDDDK tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) -
Biological activity
Cereblon-BRD3 interaction was assessed using A-screen technology. Various amounts of ab271467 were incubated in 10-ul reaction with dBET1 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
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Purity
>= 90 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
AGEGDQQDAA HNMGNHLPLL PAESEEEDEM EVEDQDSKEA KKPNIINFDT SLPTSHTYLG ADMEEFHGRT LHDDDSCQVI PVLPQVMMIL IPGQTLPLQL FHPQEVSMVR NLIQKDRTFA VLAYSNVQER EAQFGTTAEI YAYREEQDFG IEIVKVKAIG RQRFKVLELR TQSDGIQQAK VQILPECVLP STMSAVQLES LNKCQIFPSK PVSREDQCSY KWWQKYQKRK FHCANLTSWP RWLYSLYDAE TLMDRIKKQL REWDENLKDD SLPSNPIDFS YRVAACLPID DVLRIQLLKI GSAIQRLRCE LDIMNKCTSL CCKQCQETEI TTKNEIFSLS LCGPMAAYVN PHGYVHETLT VYKACNLNLI GRPSTEHSWF PGYAWTVAQC KICASHIGWK FTATKKDMSP QKFWGLTRSA LLPTIPDTED EISPDKVILC L -
Predicted molecular weight
51 kDa including tags -
Amino acids
2 to 442 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
GenBank: NM_016302
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Amino Acid Sequence 2
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Species
Human -
Sequence
MSYNYVVTAQ KPTAVNGCVT GHFTSAEDLN LLIAKNTRLE IYVVTAEGLR PVKEVGMYGK IAVMELFRPK GESKDLLFIL TAKYNACILE YKQSGESIDI ITRAHGNVQD RIGRPSETGI IGIIDPECRM IGLRLYDGLF KVIPLDRDNK ELKAFNIRLE ELHVIDVKFL YGCQAPTICF VYQDPQGRHV KTYEVSLREK EFNKGPWKQE NVEAEASMVI AVPEPFGGAI IIGQESITYH NGDKYLAIAP PIIKQSTIVC HNRVDPNGSR YLLGDMEGRL FMLLLEKEEQ MDGTVTLKDL RVELLGETSI AECLTYLDNG VVFVGSRLGD SQLVKLNVDS NEQGSYVVAM ETFTNLGPIV DMCVVDLERQ GQGQLVTCSG AFKEGSLRII RNGIGIHEHA SIDLPGIKGL WPLRSDPNRE TDDTLVLSFV GQTRVLMLNG EEVEETELMG FVDDQQTFFC GNVAHQQLIQ ITSASVRLVS QEPKALVSEW KEPQAKNISV ASCNSSQVVV AVGRALYYLQ IHPQELRQIS HTEMEHEVAC LDITPLGDSN GLSPLCAIGL WTDISARILK LPSFELLHKE MLGGEIIPRS ILMTTFESSH YLLCALGDGA LFYFGLNIET GLLSDRKKVT LGTQPTVLRT FRSLSTTNVF ACSDRPTVIY SSNHKLVFSN VNLKEVNYMC PLNSDGYPDS LALANNSTLT IGTIDEIQKL HIRTVPLYES PRKICYQEVS QCFGVLSSRI EVQDTSGGTT ALRPSASTQA LSSSVSSSKL FSSSTAPHET SFGEEVEVHN LLIIDQHTFE VLHAHQFLQN EYALSLVSCK LGKDPNTYFI VGTAMVYPEE AEPKQGRIVV FQYSDGKLQT VAEKEVKGAV YSMVEFNGKL LASINSTVRL YEWTTEKELR TECNHYNNIM ALYLKTKGDF ILVGDLMRSV LLLAYKPMEG NFEEIARDFN PNWMSAVEIL DDDNFLGAEN AFNLFVCQKD SAATTDEERQ HLQEVGLFHL GEFVNVFCHG SLVMQNLGET STPTQGSVLF GTVNGMIGLV TSLSESWYNL LLDMQNRLNK VIKSVGKIEH SFWRSFHTER KTEPATGFID GDLIESFLDI SRPKMQEVVA NLQYDDGSGM KREATADDLI KVVEELTRIH -
Predicted molecular weight
128 kDa including tags -
Amino acids
1 to 1140 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
GenBank: NM_001923
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Amino Acid Sequence 3
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Species
Human -
Sequence
ADEAPRKGSF SALVGRTNGL TKPAALAAAP AKPGGAGGSK KLVIKNFRDR PRLPDNYTQD TWRKLHEAVR AVQSSTSIRY NLEELYQAVE NLCSHKVSPM LYKQLRQACE DHVQAQILPF REDSLDSVLF LKKINTCWQD HCRQMIMIRS IFLFLDRTYV LQNSTLPSIW DMGLELFRTH IISDKMVQSK TIDGILLLIE RERSGEAVDR SLLRSLLGML SDLQVYKDSF ELKFLEETNC LYAAEGQRLM QEREVPEYLN HVSKRLEEEG DRVITYLDHS TQKPLIACVE KQLLGEHLTA ILQKGLDHLL DENRVPDLAQ MYQLFSRVRG GQQALLQHWS EYIKTFGTAI VINPEKDKDM VQDLLDFKDK VDHVIEVCFQ KNERFVNLMK ESFETFINKR PNKPAELIAK HVDSKLRAGN KEATDEELER TLDKIMILFR FIHGKDVFEA FYKKDLAKRL LVGKSASVDA EKSMLSKLKH ECGAAFTSKL EGMFKDMELS KDIMVHFKQH MQNQSDSGPI DLTVNILTMG YWPTYTPMEV HLTPEMIKLQ EVFKAFYLGK HSGRKLQWQT TLGHAVLKAE FKEGKKEFQV SLFQTLVLLM FNEGDGFSFE EIKMATGIED SELRRTLQSL ACGKARVLIK SPKGKEVEDG DKFIFNGEFK HKLFRIKINQ IQMKETVEEQ VSTTERVFQD RQYQIDAAIV RIMKMRKTLG HNLLVSELYN QLKFPVKPGD LKKRIESLID RDYMERDKDN PNQYHYVA -
Predicted molecular weight
88 kDa including tags -
Amino acids
2 to 729 -
Tags
His tag N-Terminus -
Additional sequence information
Gen Bank: NM_003589
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Amino Acid Sequence 4
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Species
Human -
Sequence
AAAMDVDTPS GTNSGAGKKR FEVKKWNAVA LWAWDIVVDN CAICRNHIMD LCIECQANQA SATSEECTVA WGVCNHAFHF HCISRWLKTR QVCPLDNREW EFQKYGH -
Amino acids
2 to 108 -
Tags
His tag N-Terminus -
Additional sequence information
GenBank: NM_014248
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Description
Recombinant human DDB1 + Cullin 4A/CUL-4A + RBX1 + CRBN protein (Active)
Preparation and Storage
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
Co-expressed in HEK-293 cells.
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Concentration information loading...
Images
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Functional Studies - Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) (ab271467)
Cereblon-BRD3 interaction.
Various amounts of ab271467 were incubated in 10-ul reaction with dBET1 and GST-BRD3(BD2) at room temperature for one hour. After this, FLAG-acceptor beads and GSH-donor beads were added followed by detection of A-counts.
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SDS-PAGE - Recombinant Human CRBN + DDB1 + CUL-4A + RBX1 protein (Tagged) (ab271467)SDS-PAGE analysis of ab271467.