Recombinant Human Cathepsin D protein (His tag) (ab276483)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 97% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
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Product name
Recombinant Human Cathepsin D protein (His tag) -
Purity
> 97 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Full length proteinAnimal free
NoNature
Recombinant-
Species
Human -
Sequence
MQPSSLLPLA LCLLAAPASA LVRIPLHKFT SIRRTMSEVG GSVEDLIAKG PVSKYSQAVP AVTEGPIPEV LKNYMDAQYY GEIGIGTPPQ CFTVVFDTGS SNLWVPSIHC KLLDIACWIH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY LSQDTVSVPC QSASSASALG GVKVERQVFG EATKQPGITF IAAKFDGILG MAYPRISVNN VLPVFDNLMQ QKLVDQNIFS FYLSRDPDAQ PGGELMLGGT DSKYYKGSLS YLNVTRKAYW QVHLDQVEVA SGLTLCKEGC EAIVDTGTSL MVGPVDEVRE LQKAIGAVPL IQGEYMIPCE KVSTLPAITL KLGGKGYKLS PEDYTLKVSQ AGKTLCLSGF MGMDIPPPSG PLWILGDVFI GRYYTVFDRD NNRVGFAEAA RL -
Predicted molecular weight
44 kDa including tags -
Molecular weight information
The secreted recombinant human CTSD (pro form) consists of 405 amino acids and has a predicted molecular mass of 44 kDa. As a result of different glycosylation, the apparent molecular mass of rhCTSD is approximately 40-110 kDa in SDS-PAGE under reducing conditions. -
Amino acids
1 to 412 -
Tags
His tag C-Terminus -
Additional sequence information
Predicted N-terminus: Ser 19.
Specifications
Our Abpromise guarantee covers the use of ab276483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 6.5
Constituents: 0.49% MES, 0.87% Sodium chloride -
ReconstitutionThis information is lot specific. Please contact our technical Support team for details.
General Info
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Alternative names
- CLN10
- CPSD
- CTSD
- Lysosomal aspartyl protease
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Relevance
Cathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N linked oligosaccharides. Cathepsin D is synthesized as an inactive pro enzyme. Activation involves the proteolytic removal of the 43 amino acid profragment and an internal cleavage to generate the two chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer and possibly Alzheimer's disease. -
Cellular localization
Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Images
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SDS-PAGE - Recombinant Human Cathepsin D protein (ab276483)SDS-PAGE analysis of ab276483
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab276483 has not yet been referenced specifically in any publications.
Preparation and Storage
- CLN10
- CPSD
- CTSD
- Lysosomal aspartyl protease
Images
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SDS-PAGE - Recombinant Human Cathepsin D protein (ab276483)SDS-PAGE analysis of ab276483