Recombinant human CARM1 protein (ab196401)
Key features and details
- Expression system: Cell Culture
- Purity: >= 65% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human CARM1 protein
See all CARM1 proteins and peptides -
Biological activity
Assay Conditions:
50 µl reaction mix (1x HMT Assay Buffer 5, and 5-1000 ng ab196401) is added to wells coated with the substrate (black plate). Incubate at room temperature for 1 hour. Add antibody against methylated R3 residue of histone H4, incubate 1 hour. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence. -
Purity
>= 65 % SDS-PAGE. -
Expression system
Cell Culture -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
AAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTIGDANGEIQRHA EQQALRLEVRAGPDSAGIALYSHEDVCVFKCSVSRETECSRVGKQSFIIT LGCNSVLIQFATPNDFCSFYNILKTCRGHTLERSVFSERTEESSAVQYFQ FYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFF AAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQV DIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQ LYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAK SVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMT VWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDIS IVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGS TYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLANTGIVNHTHSRM GSIMSTGIVQGSSGAQGSGGGSTSAHYAVNSQFTMGGPAISMASPMSIPT NTMHYGS -
Molecular weight information
66 k Da by SDS-PAGE -
Amino acids
2 to 608 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
Expressed in FreeStyle 293-F cells.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
80 ng/µL DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Active human PRMT4 full length protein (ab196401)
SDS-PAGE analysis of ab196401.
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Functional Studies - Active human PRMT4 full length protein (ab196401)Specific activity of ab196401.