Human TIMP2 ELISA Kit (ab270213)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 19.5 pg/ml
- Range: 78.125 pg/ml - 5000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TIMP2 ELISA Kit
See all TIMP2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.6% Inter-assay Sample n Mean SD CV% Serum 3 6.4% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
19.5 pg/ml -
Range
78.125 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 107 105% - 110% Saliva 109 106% - 112% Urine 101 96% - 105% Serum 108 107% - 112% Cell culture media 84 82% - 85% Hep Plasma 99 95% - 103% EDTA Plasma 109 105% - 113% Cit plasma 111 109% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TIMP2 ELISA Kit (ab270213) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TIMP2 protein in cell culture supernatant, edta plasma, saliva, serum, urine, cit plasma, hep plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TIMP2 with 19.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
TIMP2 (tissue inhibitor of metalloproteinases 2) is a member of the TIMP gene family that are known to be natural inhibitors of the matrix metalloproteinases. Moreover, TIMP2 has a unique function in its ability to suppress the proliferation of endothelial cells in response to angiogenic factors as well as inhibiting protease activity in tissues undergoing extracellular matrix remodeling. As a result, making TIMP2 critical to the maintenance of tissue homeostasis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TIMP2 Capture Antibody 1 x 600µl 10X Human TIMP2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human TIMP2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Post-translational
modificationsThe activity of TIMP2 is dependent on the presence of disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSC 21K
- CSC-21K
- CSC21K
see all -
Database links
- Entrez Gene: 7077 Human
- Omim: 188825 Human
- SwissProt: P16035 Human
- Unigene: 633514 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human TIMP2 competitor curve comparisonStandard Curve comparison between human TIMP2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human TIMP2 standard curve comparison -
Example of human TIMP2 standard curve in Sample Diluent NS.The TIMP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TIMP2 in human serum and plasma samples.The concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 15%, plasma (citrate) 15%, plasma (EDTA) 15%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 25,723 pg/mL in serum, 30,263 pg/mL in plasma (citrate), 22,927 pg/mL in plasma (EDTA), and 17,596 pg/mL in plasma (heparin).
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Interpolated concentrations of native TIMP2 in human urine and saliva samples.The concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 100%, saliva 12.5%, HepG2 supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 3,088 pg/mL in urine, 5,618 pg/mL in saliva, and 8,400 pg/mL in HepG2 supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 27,721 pg/mL with a range of 19,755 – 41,782 pg/mL.
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Sandwich ELISA - Human TIMP2 ELISA Kit (ab270213)To learn more about the advantages of recombinant antibodies see here.
