Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human TIMP2 ELISA Kit (ab270213)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 19.5 pg/ml
  • Range: 78.125 pg/ml - 5000 pg/ml
  • Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human TIMP2 ELISA Kit
    See all TIMP2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 4.6%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6.4%

  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    19.5 pg/ml

  • Range

    78.125 pg/ml - 5000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 107 105% - 110%
    Saliva 109 106% - 112%
    Urine 101 96% - 105%
    Serum 108 107% - 112%
    Cell culture media 84 82% - 85%
    Hep Plasma 99 95% - 103%
    EDTA Plasma 109 105% - 113%
    Cit plasma 111 109% - 113%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human TIMP2 ELISA Kit (ab270213) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TIMP2 protein in cell culture supernatant, edta plasma, saliva, serum, urine, cit plasma, hep plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TIMP2 with 19.5 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    TIMP2 (tissue inhibitor of metalloproteinases 2) is a member of the TIMP gene family that are known to be natural inhibitors of the matrix metalloproteinases. Moreover, TIMP2 has a unique function in its ability to suppress the proliferation of endothelial cells in response to angiogenic factors as well as inhibiting protease activity in tissues undergoing extracellular matrix remodeling. As a result, making TIMP2 critical to the maintenance of tissue homeostasis.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Human TIMP2 competitor curve comparison
    Human TIMP2 competitor curve comparison

    Standard Curve comparison between human TIMP2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Human TIMP2 standard curve comparison
    Human TIMP2 standard curve comparison

    Standard Curve comparison between the original Human MMP3 SimpleStep ELISA kit (ab188395) and current Human MMP3 SimpleStep ELISA kit (ab188395).

  • Example of human TIMP2 standard curve in Sample Diluent NS.
    Example of human TIMP2 standard curve in Sample Diluent NS.

    The TIMP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native TIMP2 in human serum and plasma samples.
    Interpolated concentrations of native TIMP2 in human serum and plasma samples.

    The concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 15%, plasma (citrate) 15%, plasma (EDTA) 15%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 25,723 pg/mL in serum, 30,263 pg/mL in plasma (citrate), 22,927 pg/mL in plasma (EDTA), and 17,596 pg/mL in plasma (heparin).

  • Interpolated concentrations of native TIMP2 in human urine and saliva samples.
    Interpolated concentrations of native TIMP2 in human urine and saliva samples.

    The concentrations of TIMP2 were measured in duplicates, interpolated from the TIMP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 100%, saliva 12.5%, HepG2 supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 3,088 pg/mL in urine, 5,618 pg/mL in saliva, and 8,400 pg/mL in HepG2 supernatant.

  • Serum from ten individual healthy human female donors was measured in duplicate.
    Serum from ten individual healthy human female donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP2 concentration was determined to be 27,721 pg/mL with a range of 19,755 – 41,782 pg/mL.

  • Sandwich ELISA - Human TIMP2 ELISA Kit (ab270213)
    Sandwich ELISA - Human TIMP2 ELISA Kit (ab270213)
    To learn more about the advantages of recombinant antibodies see here.

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