Recombinant human CaMKII beta protein (ab132961)
Key features and details
- Expression system: Wheat germ
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, WB, ELISA, Functional Studies
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Product name
Recombinant human CaMKII beta protein
See all CaMKII beta proteins and peptides -
Expression system
Wheat germ -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKL SARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFE DIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKG AAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWAC GVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLIN QMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKL KGAILTTMLATRNFSAAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPP AALESSDSANTTIEDEDAKARKQEIIKTTEQLIEAVNNGDFEAYAKICDP GLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGED AACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWQNVHFHCSGAPVA PLQ -
Predicted molecular weight
81 kDa including tags -
Amino acids
1 to 503 -
Tags
GST tag N-Terminus
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Preparation and Storage
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Alternative names
- Calcium/calmodulin dependent protein kinase (CaM kinase) II beta
- Calcium/calmodulin dependent protein kinase II beta
- Calcium/calmodulin dependent protein kinase IIB
see all -
Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
Tissue specificity
Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. - Information by UniProt
Images
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SDS-PAGE - Recombinant human CaMKII beta protein (ab132961)12.5% SDS-PAGE analysis of ab132961 stained with Coomassie Blue.
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Functional Studies - Recombinant human CaMKII beta protein (ab132961)Kinase Assay using ab132961.
Fig.1: SDS-PAGE gel
Fig.2: Autoradiography of SDS-PAGE gel.
Kinase substrates:
H3 histone, family 3A - calf thymus - Molecular weight: approx. 16.5-17 kDa on SDS-PAGE
Myelin basic protein - bovine brain - Molecular weight: approx. 21-22 kDa on SDS-PAGE
Histone H1.0 - calf thymus - Molecular weight: approx. 32-33 kDa on SDS-PAGE
Beta-casein protein- bovine milk - Molecular weight: approx. 33-34 kDa on SDS-PAGE