Recombinant human BCMA protein (Active) (ab269983)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 82% SDS-PAGE
- Active: Yes
- Tags: Avi tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human BCMA protein (Active)
See all BCMA proteins and peptides -
Biological activity
Assay performed using a BCMA:APRIL[B] assay kit. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein (BCMA) was added overnight at 4 deg 100 ng/well. Various amounts of biotinylated ligand (APRIL[B]) (0-20 ng) added for 2h at RT. Strep-HRP added for 1h at RT
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Purity
> 82 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
34 kDa including tags -
Amino acids
1 to 54 -
Tags
Avi tag C-Terminus -
Additional sequence information
C-terminal Avi tag fused to human IgG1 Fc.. NM_001192
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Preparation and Storage
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Alternative names
- B cell maturation antigen
- B cell maturation factor
- B cell maturation protein
see all -
Function
Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. -
Tissue specificity
Expressed in mature B-cells, but not in T-cells or monocytes. -
Involvement in disease
Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2. -
Sequence similarities
Contains 1 TNFR-Cys repeat. -
Cellular localization
Cell membrane. Endomembrane system. Perinuclear Golgi-like structures. - Information by UniProt
Images
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Functional Studies - (ab269983)
Functional analysis of ab269983.
Assay performed using a BCMA:APRIL[B] assay kit. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein (BCMA) was added overnight at 4 deg 100 ng/well. Various amounts of biotinylated ligand (APRIL[B]) (0-20 ng) added for 2h at RT. Strep-HRP added for 1h at RT.
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SDS-PAGE - (ab269983)SDS-PAGE analysis of ab269983 (3 μg) on a 4-20% gel with Coomassies staining.
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Size Exclusion Chromatography - Recombinant human BCMA protein (Active) (ab269983)Aggregation analysis of ab269983.