Recombinant human BACE1 protein (Active) (ab168075)
Key features and details
- Expression system: Insect cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
-
Product name
Recombinant human BACE1 protein (Active)
See all BACE1 proteins and peptides -
Biological activity
Determined using a ß-Secretase Activity Assay Kit. Specific units > 120x103 units/mg. One unit is defined as the amount of enzyme that produces 1 pmol/min EDANS by hydrolyzing the substrate BVT9964 (NH2-Arg-Glu(EDANS)-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)-Arg-COOH) at 37°C in 100 mM acetate buffer pH 4.0. -
Purity
> 90 % SDS-PAGE. -
Expression system
Insect cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
47 kDa -
Amino acids
46 to 460 -
Additional sequence information
Mature protease domain.
-
Preparation and Storage
-
Alternative names
- APP beta secretase
- Asp 2
- ASP2
see all -
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
Sequence similarities
Belongs to the peptidase A1 family. -
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
Images
-
Functional Studies - Recombinant human BACE1 protein (Active) (ab168075)
Example of BACE1 Activity Assay.
-
SDS-PAGE - Recombinant human BACE1 protein (Active) (ab168075)SDS-PAGE analysis of ab168075.
